ogr_intro
SPF
TechnolA3gia
Vonalak
Riporter
Bemutatkozás SPF Technológia Technológia Vonalak Riporter egerek
   
  Vonalak
 
 
  Design:
 
   
 
 
Transzgén/VonalJax azonosítóPubmed azonosítóKontakt személyVonal eredeti tulajdonosa
FVB/Anthttps://www.jax.org/strain/004828 http://www.ncbi.nlm.nih.gov/pubmed/17083330 Erdélyi Ferenc  
This strain originated on a FVB;129P2 background, and was
backcrossed for 11 generations on the FVB background. 
BAC_pva/gfp 2 12177202 Szabó Gábor Hannah Monyer* 
Pva-gfp transgenic mice express gfp in parvalbumin-expressing neurons and muscle.
* BAC construct was designed by AH Meyer in Monyer's lab. Pronuclear microinjection was carried out in IEM-HAS. 
calb1/folA-egfp-cre Tm023531  Hájos Norbert  
cre is expressed in neurons expressing calbindin 
camK2A/gfp 22  Szabó Gábor Szabó Gábor 
CamK2A-gfp transgenic mice express gfp in piramidal and purkinje cells.
Construct and microinjection were carried out in IEM-HAS. 
cck/ires-cre Tm012706  Hájos Norbert  
cre is expressed in both glutamatergic and GABAergic neurons
containing CCK  
crh/ires-cre Tm012704 21943598  Tóth Máté Z. Josh Huang, Cold Spring Harbor Laboratory 
 
D2 (f) Tm- 23524969 Gereben Balázs AC Bianco, Miami FL, Gereben B. 
floxolt kettes-típusú dejodáz enzim 
D3 (f) Tm- - Gereben Balázs D. Salvatore, Nápoly 
floxolt hármas-típusú dejodáz enzim 
DAT/IRES-cre Tm  Hangya Balázs  
 
dlx5/6/flpe Tg010815  Hájos Norbert  
dlx5/6-flpe may be used in intersect strategy.  
fos/cre-ERT2 Tm021882  Hájos Norbert  
cre is expressed under the control of fos in a tamoxifen-
dependent manner. 
gad65_3e/gfp 5.5 30 15115742 Szabó Gábor Szabó Gábor 
gad65-gfp transgenic mice express gfp in interneurons under the control of gad2 promoter and 5.3 kb length upstream region. 
gluRa2 (f) Tm 17296559 Hájos Norbert Hannah Monyer 
floxed-GluR1 mouse strain, Fuchs et al., 2007 Neuron 
gluRd2 (f) Tm 17296559 Hájos Norbert Hannah Monyer 
floxed-GluR4 mouse strain, Fuchs et al., 2007 
Gt(ROSA)26Sor_CAG/LSL_tdTomato Tm007905 20023653 Erdélyi Ferenc Hongkui Zeng, Allen Institute for Brain Science (AIBS) 
These Ai9 mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato), and may be useful as a Cre reporter strain. TdTomato is expressed following Cre-mediated recombination.  
Gt(ROSA)26Sor_CAG/LSL_ZsGreen1 Tm007906 20023653 Erdélyi Ferenc Hongkui Zeng, Allen Institute for Brain Science (AIBS) 
These Ai6 mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven enhanced green fluorescent protein (ZsGreen1), and may be useful as a Cre reporter strain. ZsGreen1 is expressed following Cre-mediated recombination. 
Gt(ROSA)26Sor/CAG/GCaMP3 Tm014538 22378886 Erdélyi Ferenc Hongkui Zeng, Allen Institute for Brain Science (AIBS) 
Ai38 mice express the fluorescent calcium indicator protein GCaMP3 after exposure to Cre recombinase. Following subsequent calcium binding (such as neuronal activation), bright EGFP fluorescence is observed. 
GT(ROSA)26Sor/Cre - 18799753 Erdélyi Ferenc  
The Cre Deleter mouse was developed by Artemis Pharmaceuticals (now Taconic). The mutant was created by a targeted mutation knock-in of Cre into the Gt(ROSA)26 locus. Cre is expressed under the control of the Gt(ROSA)26Sor gene. 
nestin/cre_ERT2 Tg 18160632 Fekete Csaba G Fishell, New York, NY 
Tamoxifen-indukálható Cre rekombináz nestin promóter alatt 
PSEN1//App_swe//tauP301L TmTgTg34830-JAX  Hangya Balázs  
B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax
3xTg-AD 
pva/IRES_cre -017320 17190606 Szabó Gábor Silvia Arber 
PV-Cre knockin mice express Cre recombinase in parvalbumin-expressing neurons (such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia), without disrupting endogenous Pvalb expression. 
rax/cre-ert2 Tg  Fekete Csaba Seth Blackshaw 
Az állat a Rax promoter alatt tamoxifen indukálható Cre-t termel. Felnõtt állatban az expresszió tanicita specifikus. 
sb/cag/yfp/sb 27  Erdélyi Ferenc Szabó Gábor 
Sleeping Beauty-CAG-Venus construct was made by Lajos Mátes (BRC). Transgene is integrated in one copy. Fluorescent protein expression can be found in endothelial cells and in Purkinje cells in brain.  
Tau/LSL/mgfp_iresNlacZ TmB6;129P2-Mapttm2Arbr/J  15836427 Fekete Csaba Silvia Arber 
Description
In these TaumGFP mice, Cre recombinase activity induces expression of membrane bound green fluorescent protein (mGFP) and nuclear targeted beta-galactosidase in neurons. mGFP, MARCKS ("myristoylated alanine-rich C-kinase substrate") protein fused to green fluorescent protein, targets GFP expression to the membrane, and IRES-NLS-lacZ targets expression of beta-galactosidase to the nucleus. A floxed STOP cassette prevents expression of the reporter genes until removed by Cre recombinase activity. The construct replaces the endogenous start codon in exon 2. Homozygotes are viable and fertile.

Development
A targeting vector designed by Drs. Silvia Arber and Markus Sigrist (Biozentrum, University of Basel) containing floxed STOP sequence, membrane-targeted green fluorescent protein, nuclear targeted beta-galactosidase and polyadenylation signal sequence (loxP-STOP-loxP-mGFP-IRES-NLS-lacZ-pA) was inserted into exon 2. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to C57BL/6JRccHsd mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.  
Thy1/cre TgThy1/cre (Tg)   Rózsa Balázs 
 
vglut1/cre Tm023527  Hájos Norbert  
cre is expressed spacifically in forebrain glutamatergic
neurons